Ozone (O3) is an unstable gas comprising three atoms of oxygen. It is unstable because the gas will readily degrade back to its stable state, diatomic oxygen (O2) with the formation of free oxygen atoms or free radicals. The free oxygen atoms or radicals are highly reactive and they will oxidize almost anything (including viruses, bacteria, organic and inorganic compounds) in contacts, making ozone an enormously powerful disinfectant and oxidizer.
In fact, ozone is a much stronger oxidizer than other common disinfectants such as chlorine and hypochlorite. The usage of chlorine or hypochlorite in many countries has been decreased significantly due to the possibility formation of carcinogenic by-products such as trihalomethanes (THM) during the disinfection process. In contrast, ozone disinfection does not produce any harmful residues, and all the residual ozone will be converted back to oxygen within a short time. Ozone is therefore considered as an environmentally friendly disinfectant.
In order to test for the effectiveness of ozone in reducing airborne bacteria, a clean room with area about 12m2 was selected for testing. As high level of ozone is required to kill viruses, bacteria and spores, the disinfection process was carried out when humans, animals and plants were evacuated. Depends on the size of the room, an ozone generator with 1000 mg/hr output was chosen. The capacity of the chosen ozone generator has the ability to maintain high concentration of ozone (0.5 – 5 ppm) inside. Circulation fan was placed in the room to ensure good distribution of ozone. After closing all the windows and doors, the ozone generator was turned on by remote device located outside to begin ozonation process.
Experimental data shows that ozone is effective in reducing airborne bacteria of unoccupied room. Over 90% of airborne bacteria could be reduced after ozonation. As viruses are generally more susceptible to ozone than bacteria, it could assume that all viruses are killed if large percentage of airborne bacteria are removed. Ozone is a gas that has good penetration capacity and powerful oxidizing power, thus its disinfection efficiency is superior to UV radiation and HEPA filter.